Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells

作者：Jiaqi Zhou, Haoyang Wan, Zi-Xuan Wang, Nan Jiang · 发表于：World Journal of Stem Cells · 年份：2023 · DOI：10.4252/wjsc.v15.i5.369 · 被引用次数：14 · 研究领域：Mesenchymal stem cell research、Extracellular vesicles in disease、Periodontal Regeneration and Treatments

Introduction: Transforming growth factor-beta (TGF-β) is known as standard chondrogenic differentiation agent, even though it comes with undesirable side effects such as early hypertrophic maturation, mineralization, and secretion of inflammatory/angiogenic factors.On the other hand, platelet-rich plasma (PRP) is found to have a chondrogenic impact on mesenchymal stem cell proliferation and differentiation, with no considerable side effects.Therefore, we compared chondrogenic impact of TGF-β and PRP on adipose-derived stem cells (ADSCs), to see if PRP could be introduced as an alternative to TGF-β.Methods: Differentiation of ADSCs was monitored using a couple of methods including glycosaminoglycan production, miRNAs expression, vascular endothelial growth factor (VEGF)/ tumor necrosis factor alpha (TNFα) secretion, alkaline phosphatase (ALP) and calcium content assays.Results: Accordingly, the treatment of differentiating cells with 5% (v/v) PRP resulted in higher glycosaminoglycan production, enhanced SOX9 transcription, and lowered TNFα and VEGF secretion compared to the control and TGF-β groups.Besides, the application of PRP to the media up-regulated miR-146a and miR-199a in early and late stages of chondrogenesis, respectively.Conclusion: PRP induces in vitro chondrogenesis, as well as TGF-β with lesser inflammatory and hypertrophic side effects.