Effects of dietary Clostridium butyricum on the growth performance and intestinal health of hybrid groupers (Epinephelus lanceolatus♂ × E. fuscoguttatus♀)

作者：Keng Yang, Pengwei Xun, Jianbin Huang, Kui Jiang, Xiaolin Huang, Wei Yu, Chuangwen Xu, Heizhao Lin · 发表于：Aquaculture Reports · 年份：2023 · DOI：10.1016/j.aqrep.2023.101588 · 被引用次数：12 · 研究领域：Aquaculture disease management and microbiota、Aquaculture Nutrition and Growth、Animal Nutrition and Physiology

This trial was performed to investigate the effects of Clostridium butyricum on growth and intestinal health of hybrid groupers (Epinephelus lanceolatus♂ × E. fuscoguttatus♀). 300 hybrid groupers were fed five diets containing C. butyricum at 0, 1 × 107, 2 × 107, 3 × 107, 4 × 107 CFU/g for 8 weeks. The results revealed that dietary C. butyricum significantly increased weight gain rate (WGR), specific growth rate (SGR) and relative feed intake (RFI) (P < 0.05). Moderate C. butyricum significantly promoted lipase (LPS), chymotrypsin (CTS), alpha amylase (α-AMS), total antioxidant capacity (T-AOC) activities and up-regulated intestinal manganese superoxide dismutase (MnSOD), catalase (CAT), glutathione peroxidase (GPx), interleukin10 (IL-10), transforming growth factorβ (TGF-β), zonula occludens protein-2 (ZO-2), zonula occludens protein-3 (ZO-3), Occludin and Claudin-3a mRNA levels, while significantly decreased malondialdehyde (MDA) content and down-regulated intestinal Kelch-like-ECH-associated protein 1 (Keap1) and interleukin 8 (IL-8) mRNA levels (P < 0.05). 1 × 107 CFU/g C. butyricum significantly augmented villus length, muscle thickness and goblet cells in intestine of hybrid groupers (P < 0.05). 1 × 107 CFU/g C. butyricum significantly increased richness and diversity of intestinal microbiota (P < 0.05) and abundance of beneficial bacteria. Meanwhile, 1 × 107 CFU/g C. butyricum decreased the risk of conditional pathogenic bacteria to invade the host.