Quantification of virus-infected cells using RNA FISH-Flow

作者：Cody J. Warren, Arturo Barbachano-Guerrero, Devra Huey, Qing Yang, Emma R. Worden-Sapper, Jens H. Kuhn, Sara L. Sawyer · 发表于：STAR Protocols · 年份：2023 · DOI：10.1016/j.xpro.2023.102291 · 被引用次数：9 · 研究领域：Animal Virus Infections Studies、Plant Virus Research Studies、Viral gastroenteritis research and epidemiology

We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA. RNA FISH-Flow probes can be synthesized to match any RNA virus genome, in either sense or anti-sense, enabling detection of genomes or replication intermediates within cells. Flow cytometry enables high-throughput analysis of infection dynamics within a population at the single cell level. For complete details on the use and execution of this protocol, please refer to Warren et al. (2022).1