Vaginal Epithelial Cell Membrane-Based Phototherapeutic Decoy Confers a “Three-in-One” Strategy to Treat against Intravaginal Infection of Candida albicans

作者：Yijing Lin, Qingqing Yin, Dongyan Tian, Xuewei Yang, Shuangshuang Liu, Xueying Sun, Qiaoying Chen, Bingqing Fang, Hui Liang, Li Li, Deli Zhuge, Haonan Wang, Cuiye Weng, Jie Xu, Chunnan Hu, Jiafeng Xie, Xufei Zhang, Linzhi Yan, Xiaosheng Lu, Fang Wang, Caixia Liu, Yunliang Hu, Mengchun Chen, Ledan Wang, Yijie Chen · 发表于：ACS Nano · 年份：2023 · DOI：10.1021/acsnano.2c12644 · 被引用次数：35 · 研究领域：Nanoplatforms for cancer theranostics、Photodynamic Therapy Research Studies、Luminescence and Fluorescent Materials

Phototherapy is an effective strategy to control Candida albicans ( C. albicans ) infection without raising the concern of drug resistance. Despite its effectiveness, a higher dose of phototherapeutic power is required for C. albicans elimination compared to bacteria that have to be used, which is readily accompanied by off-target heat and toxic singlet oxygen to damage normal cells, thus limiting its usefulness for antifungal applications. Here to overcome this, we develop a “three-in-one” biomimetic nanoplatform consisting of an oxygen-dissolved perfluorocarbon camouflaged by a photosensitizer-loaded vaginal epithelial cell membrane. With a cell membrane coating, the nanoplatform is capable of specifically binding with C. albicans at the superficial or deep vaginal epithelium, thereby centering the phototherapeutic agents on C. albicans . Meanwhile, the cell membrane coating endows the nanoplatform to competitively protect healthy cells from candidalysin-medicated cytotoxicity. Upon candidalysin sequestration, pore-forming on the surface of the nanoplatform accelerates release of the preloaded photosensitizer and oxygen, resulting in enhanced phototherapeutic power for improved anti- C. albicans efficacy under near-infrared irradiation. In an intravaginal C. albicans -infected murine model, treatment with the nanoplatform leads to a significantly decreased C. albicans burden, particularly when leveraging candidalysin for further elevated phototherapy and C. albicans inhibit...