Influence of the casing layer on the specific volatile compounds and microorganisms by Agaricus bisporus

作者：Yonghui Wang, Xiaoying Yang, Luzhang Wan, Haixia Ren, Ling Qu, Huidong Guo, Lili Dong, Xiao Lu, Pengfei Ren · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1154903 · 被引用次数：8 · 研究领域：Fungal Biology and Applications、Mycorrhizal Fungi and Plant Interactions、Chemical synthesis and alkaloids

One of the major variables affecting yield of the mushroom Agaricus bisporus is the casing layer, which directly affects the productivity and mass. Here, volatile organic compounds were extracted by headspace solid-phase microextraction and high-throughput sequencing was used to analyze the microbial community diversity. The relationship between mushroom yield at different cropping stages and the contents of volatile organic compounds and microorganisms in three different casing layers: peat, peat + soil and soil were systematically evaluated. The result shows that Benzaldehyde and (E)-2-octenal which stimulate yield, obviously increased as mushrooms grew, while 3-octanone, which inhibits yield, decreased over time in all three casing layers. However, there was not a strong correlation between the concentration of volatile compounds and yield. In addition, more than 3,000 bacterial operational taxonomic units (OTUs) by performing high throughput sequencing of the microbes were obtained in the three casing layers. Interestingly, the microbial community compositions were very similar between the three casing layers at a later cropping stage, but the community richness varied significantly in different casing layers and at different cropping stages. At the phylum level, the communities had similar structures but were quantitively very different, and this was even more obvious at the genus level. Principal component analysis revealed significant alterations in microbial community...