Kanamycin treatment in the pre-symptomatic stage of a Drosophila PD model prevents the onset of non-motor alterations

作者：Daniela Molina‐Mateo, Benjamín Valderrama, Rafaella V. Zárate, Sergio Hidalgo, Javier Tamayo-Leiva, Alondra Soto-González, S. Guerra-Ayala, V. Arriagada-Vera, Carlos Oliva, Beatriz Dı́ez, Jorge M. Campusano · 发表于：Neuropharmacology · 年份：2023 · DOI：10.1016/j.neuropharm.2023.109573 · 被引用次数：5 · 研究领域：Insect symbiosis and bacterial influences、Mosquito-borne diseases and control、Parasitic Diseases Research and Treatment

Parkinson's disease (PD) is a neurodegenerative disorder characterized by motor alterations, which is preceded by a prodromal stage where non-motor symptoms are observed. Over recent years, it has become evident that this disorder involves other organs that communicate with the brain like the gut. Importantly, the microbial community that lives in the gut plays a key role in this communication, the so-called microbiota-gut-brain axis. Alterations in this axis have been associated to several disorders including PD. Here we proposed that the gut microbiota is different in the presymptomatic stage of a Drosophila model for PD, the Pink1 B9 mutant fly, as compared to that observed in control animals. Our results show this is the case: there is basal dysbiosis in mutant animals evidenced by substantial difference in the composition of midgut microbiota in 8–9 days old Pink1 B9 mutant flies as compared with control animals. Further, we fed young adult control and mutant flies kanamycin and analyzed motor and non-motor behavioral parameters in these animals. Data show that kanamycin treatment induces the recovery of some of the non-motor parameters altered in the pre-motor stage of the PD fly model, while there is no substantial change in locomotor parameters recorded at this stage. On the other hand, our results show that feeding young animals the antibiotic , results in a long-lasting improvement of locomotion in control flies. Our data support that manipulations of gut microbiota...