Cartilage Damage Pathological Characteristics of Diabetic Neuropathic Osteoarthropathy

作者：Pei-Long Liu, Jiayu Diao, Qiong Wang, Huan Liu, Yan Zhang, Jingqi Liang, Feng Zhang, Xiaojun Liang, Hongmou Zhao · 发表于：Analytical Cellular Pathology · 年份：2023 · DOI：10.1155/2023/7573165 · 被引用次数：4 · 研究领域：Osteoarthritis Treatment and Mechanisms、Diabetic Foot Ulcer Assessment and Management、Pain Mechanisms and Treatments

Background . Diabetic neuropathic osteoarthropathy (DNOAP) is a rare and easily missed complication for diabetes that leads to increased morbidity and mortality. DNOAP is characterized by progressive destruction of bone and joint, but its pathogenesis remains elusive. We herein aimed to investigate the pathological features and pathogenesis of the cartilages damage in DNOAP patients. Methods . The articular cartilages of eight patients with DNOAP and eight normal controls were included. Masson staining and safranine O/fixed green staining (S‐O) were used to observe the histopathological characteristics of cartilage. The ultrastructure and morphology of chondrocytes were detected by electron microscopy and toluidine blue staining. Chondrocytes were isolated from DNOAP group and control group. The expression of receptor activator of nuclear factor kappaB ligand (RANKL), osteoprotegerin (OPG), interleukin‐1 beta (IL‐1 β ), interleukin‐6 (IL‐6), tumor necrosis factor‐alpha (TNF‐ α ), and Aggrecan protein was evaluated by western blot. Reactive oxygen species (ROS) levels were measured using a 2′,7′‐dichlorofluorescin diacetate (DCFH‐DA) probe. The percentage of apoptotic cells was determined by flow cytometry (FCM). The chondrocytes were cultured with different glucose concentrations to observe the expression of RANKL and OPG. Results . Compared with the control group, the DNOAP group showed fewer chondrocytes, subchondral bone hyperplasia, and structural disorder, and a large nu...