Engineering ketoreductases for the enantioselective synthesis of chiral alcohols

作者：Qiao Li, Zhiyuan Luo, Haomin Chen, Pengfei Zhang, Anming Wang, Roger A. Sheldon · 发表于：Chemical Communications · 年份：2023 · DOI：10.1039/d3cc01474f · 被引用次数：51 · 研究领域：Enzyme Catalysis and Immobilization、Metabolism and Genetic Disorders、Pharmacogenetics and Drug Metabolism

The use of engineered ketoreductases (KREDS), both as whole microbial cells and isolated enzymes, in the highly enantiospecific reduction of prochiral ketones is reviewed. The homochiral alcohol products are key intermediates in, for example, pharmaceuticals synthesis. The application of sophisticated protein engineering and enzyme immobilisation techniques to increase industrial viability are discussed.