Long-Term Nitrogen Addition Exerts Minor Effects on Microbial Community but Alters Sensitive Microbial Species in a Subtropical Natural Forest

作者：Min He, Xiaojian Zhong, Yun Xia, Linglin Xu, Quanxin Zeng, Liuming Yang, Yuexin Fan · 发表于：Forests · 年份：2023 · DOI：10.3390/f14050928 · 被引用次数：18 · 研究领域：Soil Carbon and Nitrogen Dynamics、Mycorrhizal Fungi and Plant Interactions、Microbial Community Ecology and Physiology

Increasing nitrogen (N) deposition profoundly affects nutrient cycling in soil, thereby influencing forest ecosystem productivity and function. Soil microorganisms are integral in driving nutrient turnover; the changes in microbial communities in response to N deposition and the associated soil nutrient availability, especially of limited nutrients, are far from clear. To explore the changes in soil bacterial and fungal communities and their key environmental drivers under N deposition, we conducted a multilevel field N addition experiment in a Castanopsis carlesii natural forest. Soil properties and bacterial and fungal communities were investigated. There were no significant changes in alpha diversities (presented as Chao1 and Shannon’s indexes) and beta diversities of bacteria and fungi among the three treatments. Consistently, the relative abundances of dominant bacterial phyla (i.e., Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, and Planctomycetes) and fungal phyla (i.e., Basidiomycota, Ascomycota, and Rozellomycota) did not change following N addition. These results suggest that N deposition did not alter microbial community diversity and structure. In addition, the results of the Mantel test showed that soil pH, NO3−-N, dissolved organic N (DON), and total phosphorus (TP) predominantly influenced the community diversity and structure in bacteria, but not in fungi. Meanwhile, the relative abundance of some sensitive microbial genera, such as Bryobacter, Br...