Integrated Spatial Transcriptomic and Proteomic Analysis of Fresh Frozen Tissue Based on Stereo-seq

作者：Sha Liao, Yang Heng, Weiqing Liu, Jinqiong Xiang, Yong Ma, Ligen Chen, Xiuwen Feng, Dongmei Jia, Diyan Liang, Caili Huang, Jiajun Zhang, Min Jian, Kui Su, Mei Li, Yuin‐Han Loh, Ao Chen, Xun Xu · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2023 · DOI：10.1101/2023.04.28.538364 · 被引用次数：65 · 研究领域：Single-cell and spatial transcriptomics、Immune cells in cancer、Extracellular vesicles in disease

To simultaneously detect whole transcriptomes and protein markers on the same tissue section, we combined Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) and Stereo-seq to develop the Stereo-CITE-seq workflow. Here, we demonstrated that Stereo-CITE-seq can co-detect mRNAs and proteins in immune organs with high spatial resolution, reproducibility and accuracy.