Mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease

作者：Chenguang Niu, Ye Tu, Qiaoqiao Jin, Zhanyi Chen, Keyong Yuan, Min Wang, Pengfei Zhang, Junyuan Luo, Hao Li, Yueyi Yang, Xiaoyu Liu, Mengying Mao, Ting Dong, Wenduo Tan, Xuchen Hu, Yihuai Pan, Lili Hou, Rui Ma, Zhengwei Huang · 发表于：Frontiers in Cellular and Infection Microbiology · 年份：2023 · DOI：10.3389/fcimb.2023.1157368 · 被引用次数：25 · 研究领域：Gut microbiota and health、Probiotics and Fermented Foods、Oral microbiology and periodontitis research

Metabolic dysfunction-associated fatty liver disease (MAFLD) is a phenotype of liver diseases associated with metabolic syndrome. The pathogenesis MAFLD remains unclear. The liver maintains is located near the intestine and is physiologically interdependent with the intestine via metabolic exchange and microbial transmission, underpinning the recently proposed “oral-gut-liver axis” concept. However, little is known about the roles of commensal fungi in the disease development. This study aimed to characterize the alterations of oral and gut mycobiota and their roles in MAFLD. Twenty-one MAFLD participants and 20 healthy controls were enrolled. Metagenomics analyses of saliva, supragingival plaques, and feces revealed significant alterations in the gut fungal composition of MAFLD patients. Although no statistical difference was evident in the oral mycobiome diversity within MAFLD and healthy group, significantly decreased diversities were observed in fecal samples of MAFLD patients. The relative abundance of one salivary species, five supragingival species, and seven fecal species was significantly altered in MAFLD patients. Twenty-two salivary, 23 supragingival, and 22 fecal species were associated with clinical parameters. Concerning the different functions of fungal species, pathways involved in metabolic pathways, biosynthesis of secondary metabolites, microbial metabolism in diverse environments, and carbon metabolism were abundant both in the oral and gut mycobiomes. Mor...