Carryover Contamination-Controlled Amplicon Sequencing Workflow for Accurate Qualitative and Quantitative Detection of Pathogens: a Case Study on SARS-CoV-2

作者：Lifen Gao, Lun Li, Bin Fang, Zhiwei Fang, Yanghai Xiang, Min Zhang, Junfei Zhou, Huiyin Song, Lihong Chen, Tiantian Li, Huafeng Xiao, Renjing Wan, Yongzhong Jiang, Hai Peng · 发表于：Microbiology Spectrum · 年份：2023 · DOI：10.1128/spectrum.00206-23 · 被引用次数：9 · 研究领域：Genomics and Phylogenetic Studies、Bacterial Identification and Susceptibility Testing、Biosensors and Analytical Detection

Accuracy, a key indicator of pathogen detection technology, is compromised by carryover contamination in the amplicon sequencing workflow. Taking the detection of SARS-CoV-2 as case, this study presents a new carryover contamination-controlled amplicon sequencing workflow. The new workflow significantly reduces the degree of contamination in the workflow, thereby significantly improving the accuracy and sensitivity of the SARS-CoV-2 detection and empowering the ability of quantitative detection. More importantly, the use of the new workflow is simple and economical. Therefore, the results of this study can be easily applied to other microorganism, which has great significance for improving the detection level of microorganism.