Metabolites analysis of plantamajoside based on gut microbiota-drug interaction

作者：Hui Xu, Hang Yu, Jie Fu, Zhengwei Zhang, Jia-Chun Hu, Jin‐Yue Lu, Xinyu Yang, Meng-Meng Bu, Jian‐Dong Jiang, Yan Wang · 发表于：Phytomedicine · 年份：2023 · DOI：10.1016/j.phymed.2023.154841 · 被引用次数：20 · 研究领域：Bioactive Compounds in Plants、Traditional Chinese Medicine Analysis、Neurological Disease Mechanisms and Treatments

BACKGROUND: Plantaginis Herba (Plantago asiatica L.) has the effects of clearing heat and diuresis, oozing wet and drenching. As the main active components of Plantaginis Herba (Plantago asiatica L.), plantamajoside have a wide range of antitumor activities but very low bioavailability. The process of interacting between plantamajoside and gut microbiota remains unclear. PURPOSE: To illustrate the process of interacting between plantamajoside and gut microbiota based on high-resolution mass spectrometry and targeted metabolomics methods. STUDY DESIGN AND METHODS: This experiment was divided into two parts. First, metabolites produced from plantamajoside by gut microbiota were identified and quantified based on high-resolution mass spectrometry and LC-MS/MS. Additionally, stimulation of plantamajoside on gut microbiota-derived metabolites was determined by targeted metabolomics and gas chromatography. RESULTS: We first found that plantamajoside was rapidly metabolized by gut microbiota. Then, we identified metabolites of plantamajoside by high-resolution mass spectrometry and speculated that plantamajoside was metabolized into five metabolites including calceolarioside A, dopaol glucoside, hydroxytyrosol, 3-(3-hydroxyphenyl) propionic acid (3-HPP) and caffeic acid. Among them, we quantitatively analyzed four possible metabolites based on LC‒MS/MS and found that hydroxytyrosol and 3-HPP were final products by the gut microbiota. In addition, we studied whether plantamajoside co...