An atypical NLR gene confers bacterial wilt susceptibility in Arabidopsis

作者：Choghag Demirjian, Narjes Razavi, Gang Yu, Baptiste Mayjonade, Lu Zhang, Fabien Lonjon, G Chardon, Sébastien Carrère, Jérôme Gouzy, Stéphane Genin, Alberto P. Macho, Fabrice Roux, Richard Berthomé, Fabienne Vailleau · 发表于：Plant Communications · 年份：2023 · DOI：10.1016/j.xplc.2023.100607 · 被引用次数：17 · 研究领域：Plant Pathogenic Bacteria Studies、Plant-Microbe Interactions and Immunity、Plant pathogens and resistance mechanisms

Quantitative disease resistance (QDR) remains the most prevalent form of plant resistance in crop fields and wild habitats. Genome-wide association studies (GWAS) have proved to be successful in deciphering the quantitative genetic basis of complex traits such as QDR. To unravel the genetics of QDR to the devastating worldwide bacterial pathogen Ralstonia solanacearum, we performed a GWAS by challenging a highly polymorphic local mapping population of Arabidopsis thaliana with four R. solanacearum type III effector (T3E) mutants, identified as key pathogenicity determinants after a first screen on an A. thaliana core collection of 25 accessions. Although most quantitative trait loci (QTLs) were highly specific to the identity of the T3E mutant (ripAC, ripAG, ripAQ, and ripU), we finely mapped a common QTL located on a cluster of nucleotide-binding domain and leucine-rich repeat (NLR) genes that exhibited structural variation. We functionally validated one of these NLRs as a susceptibility factor in response to R. solanacearum, named it Bacterial Wilt Susceptibility 1 (BWS1), and cloned two alleles that conferred contrasting levels of QDR. Further characterization indicated that expression of BWS1 leads to suppression of immunity triggered by different R. solanacearum effectors. In addition, we showed a direct interaction between BWS1 and RipAC T3E, and BWS1 and SUPPRESSOR OF G2 ALLELE OF skp1 (SGT1b), the latter interaction being suppressed by RipAC. Together, our results hig...