Effect of arsenic stress on the intestinal structural integrity and intestinal flora abundance of Cyprinus carpio

作者：Xiaodan Shi, Wei Xu, Xinghua Che, Jiawen Cui, Xinchi Shang, Xiaohua Teng, Zhiying Jia · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1179397 · 被引用次数：62 · 研究领域：Arsenic contamination and mitigation、Chromium effects and bioremediation、Aquaculture disease management and microbiota

Aquatic organisms such as fish can accumulate high concentrations of arsenic (As), which has toxic effects on fish. However, whether the intestinal flora are involved in As damage to fish intestinal tissues and the underlying process are unclear. Common carp (Cyprinus carpio ) were exposed to As (2.83 mg/L) in water for 30 days, and blood, muscle, intestine, and intestine samples were collected. Intestinal pathological sections were observed, and the lipopolysaccharide (LPS) levels in serum and the levels of As accumulation and tight junction-related factors in intestinal tissues were measured. The gut microbiota was analysed by 16S rRNA sequencing. The results showed that As treatment decreased the abundance of microbiota, increased the number of harmful bacteria, and decreased the number of beneficial bacteria in the intestine. In our experiment, the top 30 harmful and beneficial bacteria with the highest relative abundance were identified. Among the top 30 harmful and beneficial bacteria, As treatment resulted in a significant ( P < 0.05) increase in harmful bacteria (such as Fusobacteriota, Bacteroidota (LPS-producing bacteria), Verrucomicrobiota, Bacteroides, Aeromonas , and Stenotrophomonas ) and a significant ( P < 0.05) decrease in beneficial bacteria (such as Actinobacteriota, Planctomycetota, Firmicutes, Reyranella, Akkermansia , and Pseudorhodobacter ), which further demonstrated that As affects the abundance of intestinal flora. In addition, As expos...