Characterization of Lung and Oral Microbiomes in Lung Cancer Patients Using Culturomics and 16S rRNA Gene Sequencing

作者：Yifan Sun, Yuejiao Liu, Jianjie Li, Yafang Tan, Tongtong An, Minglei Zhuo, Zhiyuan Pan, Menglei Ma, Bo Jia, Hongwei Zhang, Ziping Wang, Ruifu Yang, Yujing Bi · 发表于：Microbiology Spectrum · 年份：2023 · DOI：10.1128/spectrum.00314-23 · 被引用次数：76 · 研究领域：Gut microbiota and health、Mycobacterium research and diagnosis、Cancer-related molecular mechanisms research

The relationship between lung microbiota and cancer has been explored based on DNA sequencing; however, culture-dependent approaches are indispensable for further studies on the lung microbiota. In this study, we applied a comprehensive approach combining culturomics and 16S rRNA gene amplicon sequencing to detect members of the microbiotas in saliva and BALF samples from patients with unilateral lobar masses. We found alterations in the microbial community of patients with lung cancer, whose diversity might be site and pathology dependent. These features may be potential bacterial biomarkers and new targets for lung cancer diagnosis and treatment. In addition, a lung and oral microbial biobank from lung cancer patients was established, which represents a useful resource for studies of host-microbe interactions.