Emerging open-channel droplet arrays for biosensing

作者：Yongchao Song, Lirong Wang, Tailin Xu, Guangyao Zhang, Xueji Zhang · 发表于：National Science Review · 年份：2023 · DOI：10.1093/nsr/nwad106 · 被引用次数：31 · 研究领域：Innovative Microfluidic and Catalytic Techniques Innovation、Microfluidic and Capillary Electrophoresis Applications、Electrowetting and Microfluidic Technologies

Open-channel droplet arrays have attracted much attention in the fields of biochemical analysis, biofluid monitoring, biomarker recognition and cell interactions, as they have advantages with regard to miniaturization, parallelization, high-throughput, simplicity and accessibility. Such droplet arrays not only improve the sensitivity and accuracy of a biosensor, but also do not require sophisticated equipment or tedious processes, showing great potential in next-generation miniaturized sensing platforms. This review summarizes typical examples of open-channel microdroplet arrays and focuses on diversified biosensing integrated with multiple signal-output approaches (fluorescence, colorimetric, surface-enhanced Raman scattering (SERS), electrochemical, etc.). The limitations and development prospects of open-channel droplet arrays in biosensing are also discussed with regard to the increasing demand for biosensors.