A point mutation in the gene encoding magnesium chelatase I subunit influences strawberry leaf color and metabolism

作者：Yang-Yang Ma, Jian-Cheng Shi, Dan-Juan Wang, Liang Xia, Wei Feng, Chunmei Gong, Lijuan Qiu, Houcheng Zhou, Kevin M. Folta, Ying‐Qiang Wen, Jiayue Feng · 发表于：PLANT PHYSIOLOGY · 年份：2023 · DOI：10.1093/plphys/kiad247 · 被引用次数：48 · 研究领域：Photosynthetic Processes and Mechanisms、Plant Stress Responses and Tolerance、Plant nutrient uptake and metabolism

Magnesium chelatase (MgCh) catalyzes the insertion of magnesium into protoporphyrin IX, a vital step in chlorophyll (Chl) biogenesis. The enzyme consists of 3 subunits, MgCh I subunit (CHLI), MgCh D subunit (CHLD), and MgCh H subunit (CHLH). The CHLI subunit is an ATPase that mediates catalysis. Previous studies on CHLI have mainly focused on model plant species, and its functions in other species have not been well described, especially with regard to leaf coloration and metabolism. In this study, we identified and characterized a CHLI mutant in strawberry species Fragaria pentaphylla. The mutant, noted as p240, exhibits yellow-green leaves and a low Chl level. RNA-Seq identified a mutation in the 186th amino acid of the CHLI subunit, a base conserved in most photosynthetic organisms. Transient transformation of wild-type CHLI into p240 leaves complemented the mutant phenotype. Further mutants generated from RNA-interference (RNAi) and CRISPR/Cas9 gene editing recapitulated the mutant phenotype. Notably, heterozygous chli mutants accumulated more Chl under low light conditions compared with high light conditions. Metabolite analysis of null mutants under high light conditions revealed substantial changes in both nitrogen and carbon metabolism. Further analysis indicated that mutation in Glu186 of CHLI does not affect its subcellular localization nor the interaction between CHLI and CHLD. However, intramolecular interactions were impaired, leading to reduced ATPase and MgCh a...