Creation of a watermelon haploid inducer line via ClDMP3 -mediated single fertilization of the central cell

作者：Xiner Chen, Yuxiu Li, Gongli Ai, Jinfan Chen, Da‐Long Guo, Zhong‐Hou Zhu, Xue‐Jie Zhu, Shujuan Tian, Jiafa Wang, Man Liu, Yuan Li · 发表于：Horticulture Research · 年份：2023 · DOI：10.1093/hr/uhad081 · 被引用次数：36 · 研究领域：Chromosomal and Genetic Variations、Plant Virus Research Studies、Plant tissue culture and regeneration

Abstract The use of doubled haploids is one of the most efficient breeding methods in modern agriculture. Irradiation of pollen grains has been shown to induce haploids in cucurbit crops, possibly because it causes preferential fertilization of the central cell over the egg cell. Disruption of the DMP gene is known to induce single fertilization of the central cell, which can lead to the formation of haploids. In the present study, a detailed method of creating a watermelon haploid inducer line via ClDMP3 mutation is described. The cldmp3 mutant induced haploids in multiple watermelon genotypes at rates of up to 1.12%. These haploids were confirmed via fluorescent markers, flow cytometry, molecular markers, and immuno-staining. The haploid inducer created by this method has the potential to greatly advance watermelon breeding in the future.