Canine Myocardial Reperfusion Injury

作者：S. E. Mitsos, Fantone Jc, K P Gallagher, Kalah Walden, P. J. Simpson, G. D. Abrams, M. Anthony Schork, B. Lucchesi · 发表于：Journal of Cardiovascular Pharmacology · 年份：1986 · DOI：10.1097/00005344-198609000-00015 · 被引用次数：97 · 研究领域：Cardiac Ischemia and Reperfusion、Cardiac Arrest and Resuscitation、Electron Spin Resonance Studies

Summary: Oxygen-derived free radicals and their metabolites may contribute to the extension of irreversible cellular injury, which occurs on reperfusion of the previously ischemic myocardium. Therefore, therapy directed against the toxic effects of reactive oxygen species may provide protection to the ischemic myocardium, which undergoes subsequent reperfusion. We evaluated the effectiveness of N-2-mercaptopropionyl glycine (MPG), a free radical scavenger, to limit the extent of irreversible injury resulting from 90 min of ischemia followed by 6 h of reperfusion in a canine model of myocardial infarction. In three groups of dogs, MPG (20 mg/kg) was administered as a constant infusion into the left atrium. Group I received MPG for 2 h, starting 15 min before occlusion of the left circumflex coronary artery and ending 15 min after reperfusion. Group II received MPG for 1 h, starting 15 min before reperfusion. Group III received MPG for 1 h beginning 45 min after reperfusion. Each group was compared with its respective saline control group. Infarct size was reduced by 35% in Group I (32.2 ± 5.1% vs. 47.7 ± 3.4% of the area at risk, p < 0.05) and Group II (31.4 ± 3.6% vs. 47.5 ± 5.1% of the area at risk, p < 0.025) in comparison with the saline treated control animals. In contrast, in Group III infarct size did not differ significantly from the saline-treated control group (45.9 ± 3.3% vs. 47.7 ± 3.5% of the area at risk). The percent of left ventricle at risk did not differ amon...