A comprehensive catalog with 100 million genes and 3,000 metagenome-assembled genomes from global cold seep sediments

作者：Yingchun Han, Chuwen Zhang, Zhuoming Zhao, Yongyi Peng, Jing Liao, Qiuyun Jiang, Qing Liu, Zongze Shao, Xiyang Dong · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2023 · DOI：10.1101/2023.04.10.536201 · 被引用次数：2 · 研究领域：Microbial Community Ecology and Physiology、Genomics and Phylogenetic Studies、Methane Hydrates and Related Phenomena

Abstract Cold seeps harbor abundant and diverse microbes that represent a tremendous potential for biological applications and also have a significant influence on biogeochemical cycles. Though recent metagenomic studies have expanded our understanding of the microbial community and function of seep microorganisms, the knowledge of diversity and genetic repertoire of global seep microbes is lacking. Here, we collected a compilation of 165 metagenomic data from 16 cold seep sites across the globe to construct comprehensive gene and genome catalogs. The non-redundant gene catalog was comprised of 147 million genes (clustered at 95% amino acid identity), and 35.72% of them could not be assigned to a function with the currently available databases. A total of 3,164 species-level representative metagenome-assembled genomes (MAGs) are obtained, most of which (94.31%) belong to novel species. Of them, 81 ANME species are identified covering all subclades except ANME-2d, and 23 syntrophic SRB species spanning Seep-SRB1a Seep-SRB1g, and Seep-SRB2 clades. The non-redundant gene and MAGs catalogs are a valuable resource that enables expanded knowledge of the structure and functions of cold seep microbiomes.