Research Note: Therapeutic effect of a Salmonella phage combination on chicks infected with Salmonella Typhimurium

作者：Guijuan Hao, Peiyong Li, Jiaqi Huang, Ketong Cui, Lu Liang, Fang Lin, Zhiyuan Lu, Shuhong Sun · 发表于：Poultry Science · 年份：2023 · DOI：10.1016/j.psj.2023.102715 · 被引用次数：19 · 研究领域：Bacteriophages and microbial interactions、Salmonella and Campylobacter epidemiology、Aquaculture disease management and microbiota

Antibiotic treatment failure is increasingly encountered for the emergence of pandrug-resistant isolates, including the prototypical broad-host-range Salmonella enterica serovar (S.) Typhimurium, which mainly transmitted to humans through poultry products. In this study we explored the therapeutic potential of a Salmonella phage composition containing a virulent phage and a nonproductive phage that does not produce progeny phage against chicks infected with a pandrug-resistant S. Typhimurium strain of avian origin. After approximately 107 CFU of S. Typhimurium strain ST149 were administrated to chicks by intraperitoneal injection, the phage combination (∼108 PFU) was gavaged at 8-h, 32-h, and 54-h postinfection. At d 10 postinfection, phage treatment completely protected chicks from Salmonella-induced death compared to 91.7% survival in the Salmonella challenge group. In addition, phage treatment also greatly reduced the bacterial load in various organs, with Salmonella colonization levels decreasing more significantly in spleen and bursa than in liver and cecal contents, possibly due to higher phage titers in these immune organs. However, phages could not alleviate the decreased body weight gain and the enlargement of spleen and bursa of infected chicks. Further examination of the bacterial flora in the cecal contents of chicks found that S. Typhimurium infection caused a remarkable decrease in abundance of Clostridia vadin BB60 group and Mollicutes RF39 (the dominant genus ...