Prognostic value of various immune cells and Immunoscore in triple-negative breast cancer

作者：Xinyu Ren, Yu Song, Junyi Pang, Longyun Chen, Liangrui Zhou, Zhiyong Liang, Huanwen Wu · 发表于：Frontiers in Immunology · 年份：2023 · DOI：10.3389/fimmu.2023.1137561 · 被引用次数：23 · 研究领域：Cancer Immunotherapy and Biomarkers、Immunotherapy and Immune Responses、Single-cell and spatial transcriptomics

Background This study aimed to evaluate the expression status and prognostic role of various immunoregulatory cells and test in triple-negative breast cancer (TNBC). Methods The expression of five markers (CD3/CD4/CD8/CD19/CD163) of tumor immune cells was evaluated retrospectively in tumor sections from 68 consecutive cases of TNBC by immunohistochemistry. Computational image analysis was used to quantify the density and distribution of each immune marker within the tumor region, tumor invasive margin, and expression hotspots. Immunoscores were calculated using an automated approach. Other clinical characteristics were also analyzed. Results For all patients, Kaplan–Meier survival analysis showed that high CD3+ signals in the tumor region (disease-free survival (DFS), P =0.0014; overall survival (OS), P= 0.0031) and total region (DFS, P= 0.0014; OS, P= 0.0031) were significantly associated with better survival. High CD4+ levels in the tumor region and total regions were significantly associated with better survival ( P< 0.05). For Hotspot analysis, CD3+ was associated with significantly better survival for all Top1, Top2, and Top3 densities (DFS and OS, P< 0.05). High CD4+ levels were significantly associated with better prognosis for Top1 and Top3 densities (DFS and OS, P< 0.05). For stage IIB and IIIC patients, CD3+ in the tumor region and all Top hotspots was found to be significantly correlated with survival (DFS and OS, P< 0.05). CD4+ cells we...