Bifunctional Single-Molecular Fluorescent Probe: Visual Detection of Mitochondrial SO2 and Membrane Potential

作者：Wenming Ai, Yingcui Bu, Houshi Huang, Junjun Wang, Mengjuan Ren, Yu Deng, Yicai Zhu, Sen Wang, Zhipeng Yu, Hongping Zhou · 发表于：Analytical Chemistry · 年份：2023 · DOI：10.1021/acs.analchem.2c05021 · 被引用次数：47 · 研究领域：Molecular Sensors and Ion Detection、Sulfur Compounds in Biology、Redox biology and oxidative stress

Mitochondrial membrane potential (MMP) and sulfur dioxide (SO 2 ) significantly affect the mitochondrial state. In this work, TC-2 and TC-8 were constructed through side-chain engineering, in which TC-2 bearing the poorer hydrophobicity could localize on mitochondria better. Interestingly, short-wave emission was captured due to the sensitive response of TC-2 to SO 2 (LOD = 13.8 nM). Meanwhile, the probe could bind with DNA, presenting enhanced long-wave emission. Encouragingly, TC-2 could migrate from mitochondria to the nucleus when MMP was decreased, accompanied by the increase of fluorescence lifetime (9-fold). Hence, TC-2 could be used for dual-channel monitoring of mitochondrial SO 2 and MMP, which showed a completely different pathway from the commercial MMP detectors JC-1/JC-10. The cellular experiments showed that MMP was gradually decreased due to reactive oxygen species-triggered oxidative stress, and the SO 2 level was up-regulated simultaneously. Overall, this work proposed a new method to investigate and diagnose the mitochondrial-related diseases.