Transcriptomic analysis reveals shared gene signatures and molecular mechanisms between obesity and periodontitis

作者：Yisheng Cai, Xuemei Zuo, Yuyang Zuo, Shuang Wu, Weiwei Pang, Keqiang Ma, Qiaorong Yi, Lijun Tan, Hong‐Wen Deng, Xiaochao Qu, Xiang‐Ding Chen · 发表于：Frontiers in Immunology · 年份：2023 · DOI：10.3389/fimmu.2023.1101854 · 被引用次数：32 · 研究领域：Oral microbiology and periodontitis research、Cancer-related molecular mechanisms research、Inflammatory mediators and NSAID effects

Background: Both obesity (OB) and periodontitis (PD) are chronic non-communicable diseases, and numerous epidemiological studies have demonstrated the association between these two diseases. However, the molecular mechanisms that could explain the association between OB and PD are largely unclear. This study aims to investigate the common gene signatures and biological pathways in OB and PD through bioinformatics analysis of publicly available transcriptome datasets. Methods: The RNA expression profile datasets of OB (GSE104815) and PD (GSE106090) were used as training data, and GSE152991 and GSE16134 as validation data. After screening for differentially expressed genes (DEGs) shared by OB and PD, gene enrichment analysis, protein-protein interaction (PPI) network construction, GeneMANIA analysis, immune infiltration analysis and gene set enrichment analysis (GSEA) were performed. In addition, receiver operating characteristic (ROC) curves were used to assess the predictive accuracy of the hub gene. Finally, we constructed the hub gene-associated TF-miRNA-mRNA regulatory network. Results: We identified a total of 147 DEGs shared by OB and PD (38 down-regulated and 109 up-regulated). Functional analysis showed that these genes were mainly enriched in immune-related pathways such as B cell receptor signalling, leukocyte migration and cellular defence responses. 14 hub genes (FGR, MNDA, NCF2, FYB1, EVI2B, LY86, IGSF6, CTSS, CXCR4, LCK, FCN1, CXCL2, P2RY13, MMP7) showed high sen...