Rapid and highly efficient morphogenic gene-mediated hexaploid wheat transformation

作者：Kari Johnson, Uyen Cao Chu, Geny Anthony, Emily Wu, Ping Che, Todd J. Jones · 发表于：Frontiers in Plant Science · 年份：2023 · DOI：10.3389/fpls.2023.1151762 · 被引用次数：32 · 研究领域：Plant tissue culture and regeneration、Transgenic Plants and Applications、Chromosomal and Genetic Variations

L.) has been reported to improve transformation by inducing rapid somatic embryo formation. Here, we report two morphogenic gene-mediated wheat transformation methods, either with or without morphogenic and marker gene excision. These methods yield independent-transformation efficiency up to 58% and 75%, respectively. In both cases, the tissue culture duration for generating transgenic plants was significantly reduced from 80 to nearly 50 days. In addition, the transformation process was significantly simplified to make the procedure less labor-intensive, higher-throughput, and more cost-effective by eliminating the requirement for embryonic axis excision, bypassing the necessity for prolonged dual-selection steps for callus formation, and obviating the prerequisite of cytokinin for shoot regeneration. Furthermore, we have demonstrated the flexibility of the methods and generated high-quality transgenic events across multiple genotypes using herbicide (phosphinothricin, ethametsulfuron)- and antibiotic (G418)-based selections.