Maslinic Acid Ameliorates Myocardial Ischemia Reperfusion Injury-Induced Oxidative Stress via Activating Nrf2 and Inhibiting NF-κB Pathways

作者：Qi Li, Zhuqing Li, Chunlei Liu, Mengping Xu, Tingting Li, Yanxin Wang, Jiaxin Feng, Xuemei Yin, Xiaoyu Du, Chengzhi Lu · 发表于：The American Journal of Chinese Medicine · 年份：2023 · DOI：10.1142/s0192415x2350043x · 被引用次数：11 · 研究领域：Natural product bioactivities and synthesis、Genomics, phytochemicals, and oxidative stress、Phytochemicals and Antioxidant Activities

Maslinic acid (MA) is a pentacyclic triterpene obtained from the peel of olives that exhibits anti-inflammatory and antioxidant properties in several conditions. Our previous study revealed that MA exerted a cardioprotective effect by repressing inflammation and apoptosis during myocardial ischemia-reperfusion injury (MIRI). However, data regarding the antioxidative effects of MA on MIRI remains limited. This study aims to elucidate the antioxidative roles and underlying mechanisms of MA on MIRI. The left anterior descending coronary artery of rats was subjected to ligate for the induction of the ischemia/reperfusion (I/R) model and the H9c2 cells were exposed to hydrogen peroxide (H 2 O 2 ) to mimic oxidative stress. The results showed that MA reduced the I/R-induced myocardial injury and H 2 O 2 -induced cardiomyocyte death in a dose-dependent manner. Meanwhile, MA increased the activities of glutathione and superoxide dismutase both in vitro and in vivo while lowering the levels of reactive oxygen species and malondialdehyde. Mechanistically, MA could facilitate Nrf2 nuclear translocation, activate the Nrf2/HO-1 signaling pathway, and repress the NF-[Formula: see text]B signaling pathway both in I/R- and H 2 O 2 -induced oxidative stress. Besides, MA promoted the intranuclear Nrf2 and HO-1 expression, which could in part be improved by QNZ (NF-[Formula: see text]B inhibitor) in H 2 O 2 -insulted cells. Conversely, MA markedly reduced the intranuclear NF-[Formula: see text]...