A Plasmonic Fluor‐Lightened Microneedle Array Enables Ultrasensitive Multitarget Whole Blood Diagnosis of Anemia in A Paper Origami‐Based Device

作者：Jing Wu, Yong Liu, Lijun Peng, Qisijing Liu, Dawei Wang, Xu Jing, Yaozhong Hu, Jing Lin, Hanyue Fu, Xuemeng Ji, Jingmin Liu, Huan Lv, Bo Peng, Bowei Zhang, Liqiong Guo, Shuo Wang · 发表于：Small · 年份：2023 · DOI：10.1002/smll.202300464 · 被引用次数：21 · 研究领域：Advancements in Transdermal Drug Delivery、Biosensors and Analytical Detection、Erythrocyte Function and Pathophysiology

Abstract This work reports a portable, origami‐type paper device with a plasmonic fluor‐labeled microneedle sensing module for the multiplexed quantification of anemia biomarkers in whole blood. Sequential steps, including serum separation, target enrichment, and multiplexed readout by a gel imager, are rapidly accomplished with the flexible and highly integrated device. The microneedle array enabled efficient sampling of trace targets from ng mL −1 to pg mL −1 level. Combined with the plasmonic fluor label, the signal is improved by ≈7.6 folds compared with the flat substrate‐based assay. The device is applied to simultaneously quantify hemoglobin (Hb), ferritin, folic acid (FA), and vitamin B 12 (VB 12 ), which are four anemia biomarkers distributed in different environments with different concentration ranges. Featured by the small sample volume (150 µL), short assay time (20 min), low cost (2 $), robust stability, and user‐friendliness, the device is promising for the rapid and accurate diagnosis of anemia in real practice.