Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics

作者：Amy E. Rochford, Alejandro Carnicer‐Lombarte, Malak Kawan, Amy Jin, Sam Hilton, Vincenzo F. Curto, Alexandra L. Rutz, Thomas Moreau, Mark Kotter, George G. Malliaras, Damiano G. Barone · 发表于：Science Advances · 年份：2023 · DOI：10.1126/sciadv.add8162 · 被引用次数：67 · 研究领域：Neuroscience and Neural Engineering、Photoreceptor and optogenetics research、Planarian Biology and Electrostimulation

The development of neural interfaces with superior biocompatibility and improved tissue integration is vital for treating and restoring neurological functions in the nervous system. A critical factor is to increase the resolution for mapping neuronal inputs onto implants. For this purpose, we have developed a new category of neural interface comprising induced pluripotent stem cell (iPSC)-derived myocytes as biological targets for peripheral nerve inputs that are grafted onto a flexible electrode arrays. We show long-term survival and functional integration of a biohybrid device carrying human iPSC-derived cells with the forearm nerve bundle of freely moving rats, following 4 weeks of implantation. By improving the tissue-electronics interface with an intermediate cell layer, we have demonstrated enhanced resolution and electrical recording in vivo as a first step toward restorative therapies using regenerative bioelectronics.