CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets

作者：Weiran Su, Junru Li, Ji Chen, Congshuo Chen, Yuzheng Wang, Huili Dai, Fengqin Li, Peifeng Liu · 发表于：Nano Research · 年份：2023 · DOI：10.1007/s12274-023-5567-4 · 被引用次数：73 · 研究领域：CRISPR and Genetic Engineering、Advanced biosensing and bioanalysis techniques、RNA and protein synthesis mechanisms

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are becoming powerful tools for disease biomarkers detection. Due to the specific recognition, cis -cleavage and nonspecific trans -cleavage capabilities, CRISPR/Cas systems have implemented the detection of nucleic acid targets (DNA and RNA) as well as non-nucleic acid targets (e.g., proteins, exosomes, cells, and small molecules). In this review, we first summarize the principles and characteristics of various CRISPR/Cas systems, including CRISPR/Cas9, Cas12, Cas13 and Cas14 systems. Then, various types of applications of CRISPR/Cas systems used in detecting nucleic and non-nucleic acid targets are introduced emphatically. Finally, the prospects and challenges of their applications in biosensing are discussed.