Rapid and simultaneous detection of viable Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio cholerae by PMA-mPCR assay in aquatic products

作者：Yuanqing Hu, Wanying Wang, Turmidzi Fath, Fengxia Li, Lin-fang Fang, Zanhu Zhou, Danfeng Zhang · 发表于：LWT · 年份：2023 · DOI：10.1016/j.lwt.2023.114663 · 被引用次数：16 · 研究领域：Vibrio bacteria research studies、Identification and Quantification in Food、Salmonella and Campylobacter epidemiology

Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio cholerae are important pathogens that pose a risk to human health. In this study, a PMA-mPCR assay was developed for detecting viable vibrios in aquatic foods. A total of 206 bacterial strains were used to evaluate the specificity. The results showed that this assay exhibited 100% specificity. The limit of detection (LOD) for the viable cells with or without PMA treatment was approximately 103 CFU/mL (1.05 × 103 CFU/mL with PMA, 1.5 × 103 CFU/mL without PMA) for V. parahaemolyticus, 103 CFU/mL (1.68 × 103 CFU/mL with PMA, 1.45 × 103 CFU/mL without PMA) for V. alginolyticus, and 103 CFU/mL (1.03 × 103 CFU/mL with PMA, 1.0 × 103 CFU/mL without PMA) for V. cholerae, reflecting that PMA treatment did not change the sensitivity. After 10 h of enrichment, the PMA-mPCR assay detected as low as 1.0 × 102 CFU/g viable V. parahaemolyticus, V. alginolyticus, and V. cholerae in spiked seafood. A total of 453 actual samples were used to assess the practical application of the assay, and the results indicated that target vibrios in seafood were accurately screened. This PMA-mPCR assay has potential as a promising tool for the rapid, reliable and simultaneous detection of viable vibrios in aquatic foods.