Drought stress in ‘Shine Muscat’ grapevine: Consequences and a novel mitigation strategy–5-aminolevulinic acid

作者：Yuxian Yang, Jiaxin Xia, Xiang Fang, Haoran Jia, Xicheng Wang, Yi-ling Lin, Siyu Liu, Mengqing Ge, Yunfeng Pu, Jinggui Fang, Lingfei Shangguan · 发表于：Frontiers in Plant Science · 年份：2023 · DOI：10.3389/fpls.2023.1129114 · 被引用次数：25 · 研究领域：Horticultural and Viticultural Research、Plant Gene Expression Analysis、Photosynthetic Processes and Mechanisms

Drought is a common and serious abiotic stress in viticulture, and it is urgent to select effective measures to alleviate it. The new plant growth regulator 5-aminolevulinic acid ( ALA ) has been utilized to alleviate abiotic stresses in agriculture in recent years, which provided a novel idea to mitigate drought stress in viticulture. The leaves of ‘Shine Muscat’ grapevine ( Vitis vinifera L.) seedlings were treated with drought (Dro), drought plus 5-aminolevulinic acid (ALA, 50 mg/L) (Dro_ALA) and normal watering (Control) to clarify the regulatory network used by ALA to alleviate drought stress in grapevine. Physiological indicators showed that ALA could effectively reduce the accumulation of malondialdehyde (MDA) and increase the activities of peroxidase (POD) and superoxide dismutase (SOD) in grapevine leaves under drought stress. At the end of treatment (day 16), the MDA content in Dro_ALA was reduced by 27.63% compared with that in Dro, while the activities of POD and SOD reached 2.97- and 5.09-fold of those in Dro, respectively. Furthermore, ALA reduces abscisic acid by upregulating CYP707A1 , thus, relieving the closure of stomata under drought. The chlorophyll metabolic pathway and photosynthetic system are the major pathways affected by ALA to alleviate drought. Changes in the genes of chlorophyll synthesis, including CHLH , CHLD , POR , and DVR ; genes related to degradation, such as CLH , SGR , PPH and PAO ; the RCA gene that is related to Rubisco; and the genes ...