Protective effect and mechanism of Polygonatum kingianum against hypoxia-induced injury

作者：Xue Li, MiJia Mei, Xuemei Pu, Xiaojiao Chen, XueFang Li, Fanying Meng, Sen He, JingPing Li, Wen Gu, Xingxin Yang, Fan Zhang, Jie Yu · 发表于：Heliyon · 年份：2023 · DOI：10.1016/j.heliyon.2023.e14353 · 被引用次数：23 · 研究领域：Traditional Chinese Medicine Analysis、Cancer, Hypoxia, and Metabolism、Flavonoids in Medical Research

Background Hypoxia is an essential cause of fatigue and aging, and is associated with the occurrence and development of many diseases. Polygonatum kingianum (PK) is a deficiency-nourishing Chinese herbal medicine utilized as both medicine and food, and it has long been used to ameliorate human conditions associated with fatigue and aging over 2000 years in China. PK is an important genuine-medicinal-materials cultivated in Yunnan, China, and is used by the Bai, Wa, and Zhuang nationalities as a traditional medicine for enhancing immunity, anti-fatigue, and anti-aging, while the preventive effect of PK on hypoxia-induced injury and the underlying mechanism are indefinite. Aim of the study The present study aimed to evaluate the anti-hypoxia efficacy and understand the corresponding mechanism of PK water extract. Materials and methods The main active ingredients and targets of PK were predicted using network pharmacology, and the anti-hypoxia activities of Gracillin and Liquiritigenin were verified by in vitro experiments. The pharmacodynamic experiments were conducted to evaluate the major signal pathways of PK for detecting anti-hypoxia activity. Results Fifty active ingredients and 371 potential targets were screened by network pharmacology, then, we confirmed that Gracillin and Liquiritigenin were the main active components of PK to exert anti-hypoxia effect in vitro. The pharmacodynamic experiments revealed that PK enhanced the extension rate of the survival time (ERST) an...