Rapid and ultrasensitive detection of acute kidney injury biomarkers CH3L1 and L-FABP using surface-enhanced Raman spectroscopy

作者：Luyao Wang, Pei Ma, Hui Chen, Min Chang, Ping Lü, Nan Chen, Xuedian Zhang, Yanhua Li, Mingxing Sui · 发表于：Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · 年份：2023 · DOI：10.1016/j.saa.2023.122604 · 被引用次数：13 · 研究领域：Acute Kidney Injury Research、Dialysis and Renal Disease Management、Spectroscopy Techniques in Biomedical and Chemical Research

Chitinase 3-like 1 (CH3L1) and liver fatty acid binding protein (L-FABP) are promising biomarkers for the early diagnosis of acute kidney injury (AKI). Here, a highly sensitive method for the quantitative detection of CH3L1 and L-FABP by surface-enhanced Raman spectroscopy (SERS) based on graphene oxide/gold and silver core–shell nanoparticles (GO/Au@Ag NPs) was proposed. The results showed that such GO/Au@Ag substrate can achieve rapid sensing of CH3L1 and L-FABP with a wide response range (2 × 10 -1 to 2 × 10 -8 mg/mL and 1.2 × 10 -1 to 1.2 × 10 -8 mg/mL, respectively) and high sensitivity. The detection limits of CH3L1 and L-FABP were 1.21 × 10 -8 mg/mL and 0.62 × 10 -8 mg/mL, respectively. In addition, the simultaneous detection of the two biomarkers in serum was demonstrated, showing the feasibility of this method in the complex biological environment. The detection of CH3L1 and L-FABP will greatly improve the early diagnosis and intervention of AKI.