The Effect of Pre-Electroacupuncture on Nociceptive Discharges of Spinal Wide Dynamic Range Neurons in Rat

作者：Q. Yu, Wan-Ying Cao, Xiaoyu Wang, Wei He, Xiaoyue Sun, Lizhen Chen, Yang‐Shuai Su, Zhiyun Zhang, Xianghong Jing · 发表于：Journal of Pain Research · 年份：2023 · DOI：10.2147/jpr.s396481 · 被引用次数：8 · 研究领域：Pain Mechanisms and Treatments、Acupuncture Treatment Research Studies、Myofascial pain diagnosis and treatment

Purpose: Spinal wide dynamic range (WDR) neurons are well studied in pain models and they play critical roles in regulating nociception. Evidence has started to accumulate that acupuncture produces a good analgesic effect via activating different primary fibers with distinct intensities. The purpose of the present study was to compare the distinct intensities of pre-electroacupuncture (pre-EA) at local muscular receptive fields (RFs), adjacent or contralateral non-RFs regulating the nociceptive discharges of spinal WDR neurons evoked by hypertonic saline (HS). Materials and Methods: Spinal segments of electrophysiological recording were identified by neural tracers applied at the left gastrocnemius muscle. The thresholds of Aβ (T Aβ ), Aδ (T Aδ ) and C (T C ) components of WDR neurons were measured to determine the intensity of pre-EA by extracellular recording. The discharges of WDR neurons induced by distinct intensities of pre-EA and 200 μL HS (6%) injection in left gastrocnemius muscle of rats were observed by extracellular recording. Results: The spinal segments of WDR neurons were confirmed in lumbar (L)5– 6 area according to the projective segments of dorsal root ganglion. T Aβ , T Aδ and T C of WDR neurons was determined to be 0.5, 1, and 2 mA, respectively. The pre-EA with intensities of T Aβ ( P < 0.05), T Aδ ( P < 0.05), T C ( P < 0.05) or 2T C ( P < 0.01) at ipsilateral adjacent non-RFs significantly reduced the discharges of WDR neurons, while at local RFs only p...