Comprehensive analysis of the laccase gene family in tea plant highlights its roles in development and stress responses

作者：Jiaxin Zhu, Hongxiu Zhang, Kelin Huang, Rui Guo, Jingjuan Zhao, Hui Xie, Junyan Zhu, Honglian Gu, Hongrong Chen, Guoqiang Li, Chaoling Wei, Shengrui Liu · 发表于：BMC Plant Biology · 年份：2023 · DOI：10.1186/s12870-023-04134-w · 被引用次数：35 · 研究领域：Plant Gene Expression Analysis、Polysaccharides and Plant Cell Walls、Plant Stress Responses and Tolerance

BACKGROUND: Laccase (LAC) is the pivotal enzyme responsible for the polymerization of monolignols and stress responses in plants. However, the roles of LAC genes in plant development and tolerance to diverse stresses are still largely unknown, especially in tea plant (Camellia sinensis), one of the most economically important crops worldwide. RESULTS: In total, 51 CsLAC genes were identified, they were unevenly distributed on different chromosomes and classified into six groups based on phylogenetic analysis. The CsLAC gene family had diverse intron-exon patterns and a highly conserved motif distribution. Cis-acting elements in the promoter demonstrated that promoter regions of CsLACs encode various elements associated with light, phytohormones, development and stresses. Collinearity analysis identified some orthologous gene pairs in C. sinensis and many paralogous gene pairs among C. sinensis, Arabidopsis and Populus. Tissue-specific expression profiles revealed that the majority of CsLACs had high expression in roots and stems and some members had specific expression patterns in other tissues, and the expression patterns of six genes by qRT‒PCR were highly consistent with the transcriptome data. Most CsLACs showed significant variation in their expression level under abiotic (cold and drought) and biotic (insect and fungus) stresses via transcriptome data. Among them, CsLAC3 was localized in the plasma membrane and its expression level increased significantly at 13 d under ...