Molecular Phylogenetic Relationships and Unveiling Novel Genetic Diversity among Slow and Pygmy Lorises, including Resurrection of Xanthonycticebus intermedius

作者：Mary E. Blair, Giang Cao, Elora H. López-Nandam, Daniel Veronese-Paniagua, Mark Birchette, Marina Kenyon, Badrul Munir Md‐Zain, Rachel A. Munds, K. A. I. Nekaris, Vincent Nijman, Christian Roos, Hoàng M. Thạch, Eleanor J. Sterling, Minh Đức Lê · 发表于：Genes · 年份：2023 · DOI：10.3390/genes14030643 · 被引用次数：22 · 研究领域：Primate Behavior and Ecology、Amphibian and Reptile Biology、Genomics and Phylogenetic Studies

Genetic analysis of historical museum collections presents an opportunity to clarify the evolutionary history of understudied primate groups, improve taxonomic inferences, and inform conservation efforts. Among the most understudied primate groups, slow and pygmy lorises (genera Nycticebus and Xanthonycticebus) are nocturnal strepsirrhines found in South and Southeast Asia. Previous molecular studies have supported five species, but studies using morphological data suggest the existence of at least nine species. We sequenced four mitochondrial loci, CO1, cytb, d-loop, and ND4, for a total of 3324 aligned characters per sample from 41 historical museum specimens for the most comprehensive geographic coverage to date for these genera. We then combined these sequences with a larger dataset composed of samples collected in Vietnam as well as previously published sequences (total sample size N = 62). We inferred phylogenetic relationships using Bayesian inference and maximum likelihood methods based on data from each locus and on concatenated sequences. We also inferred divergence dates for the most recent common ancestors of major lineages using a BEAST analysis. Consistent with previous studies, we found support for Xanthonycticebus pygmaeus as a basal taxon to the others in the group. We also confirmed the separation between lineages of X. pygmaeus from northern Vietnam/Laos/China and southern Vietnam/Cambodia and included a taxonomic revision recognizing a second taxon of pygm...