Class 1 integrons and multiple mobile genetic elements in clinical isolates of the Klebsiella pneumoniae complex from a tertiary hospital in eastern China

作者：Lan Wang, Mei Zhu, Chunxia Yan, Yanfang Zhang, Xuying He, Lin Wu, Jiefeng Xu, Junwan Lu, Qiyu Bao, Yunliang Hu, Teng Xu, Jialei Liang · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.985102 · 被引用次数：19 · 研究领域：Antibiotic Resistance in Bacteria、Bacterial biofilms and quorum sensing、Vibrio bacteria research studies

Background The emergence of highly drug-resistant K. pneumoniae , has become a major public health challenge. In this work, we aim to investigate the diversity of species and sequence types (STs) of clinical Klebsiella isolates and to characterize the prevalence and structure of class 1 integrons. Methods Based on the whole genome sequencing, species identification was performed by 16S rRNA gene homology and average nucleotide identity (ANI) analysis. STs were determined in accordance with the international MLST schemes for K. pneumoniae and K. variicola . Integron characterization and comparative genomic analysis were performed using various bioinformatic tools. Results Species identification showed that the 167 isolates belonged to four species: K. pneumoniae , K. variicola subsp. variicola , K. quasipneumoniae and K. aerogenes . Thirty-six known and 5 novel STs were identified in K. pneumoniae , and 10 novel STs were identified in K. variicola subsp. variicola . Class 1 integrons were found in 57.49% (96/167) of the isolates, and a total of 169 resistance gene cassettes encoding 19 types of resistance genes, including carbapenem resistance gene ( bla IPM-4 ) and class D β-lactamases gene ( bla OXA-1 and bla OXA-10 ), were identified. Among the 17 complete genomes, 29 class 1 integrons from 12 groups were found, only 1 group was encoded on chromosomes. Interestingly, one plasmid (pKP167-261) carrying two copies of approximately 19-kb IS 26 -Int1 complex resistance region th...