Quantitative proteomics identified a novel invasion biomarker associated with EMT in pituitary adenomas

作者：Yu Zhang, Lei Li, Xin Ma, Chenan Liu, Gemingtian Liu, Zhixu Bie, Zhijun Yang, Pinan Liu · 发表于：Frontiers in Endocrinology · 年份：2023 · DOI：10.3389/fendo.2023.1137648 · 被引用次数：6 · 研究领域：Pituitary Gland Disorders and Treatments、Ferroptosis and cancer prognosis、Clusterin in disease pathology

Background: Complete resection of invasive pituitary adenoma is usually difficult, resulting in a high recurrence rate. Therefore, it is needed to find potential diagnostic markers and therapeutic targets for invasive pituitary adenoma. Methods: We collected samples from patients with invasive and non-invasive pituitary adenomas from Beijing Tiantan Hospital for protein extraction and quantitative analysis. We identified differential proteins (DEPs) by differential analysis of the two groups. The intersection of differential proteins related to invasion and epithelial-mesenchymal transition (EMT) in the GeneCards database was identified as EMT-DEPs. The protein network of EMT-DEPs was analyzed using the STRING database and Cytoscape software, and the hub EMT-DEPs were obtained by the MCC algorithm of the cytoHubba plugin. Correlation analysis was used to obtain the interpairing proteins among EMT-DEPs, and core EMT-DEPs were identified based on the number of paired proteins. The Venn program was used to identify the intersection of hub EMT-DEPs and core EMT-DEPs as key EMT-DEPs. Finally, a series of analyses plus experiments were used to verify the correlation of the target protein with invasion and EMT in pituitary adenoma. Results: Quantitative comparison of proteins between invasive and non-invasive pituitary adenomas indicated 833 differential proteins. The overlaps of EMT-related proteins and differential proteins consisted of 46 EMT-DEPs. There were 6 intersections betw...