Exploring the differences between sole silages of gramineous forages and mixed silages with forage legumes using 16S/ITS full-length sequencing

作者：Xianjun Lai, Haiyan Wang, Junfeng Yan, Yizheng Zhang, Lang Yan · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1120027 · 被引用次数：14 · 研究领域：Ruminant Nutrition and Digestive Physiology、Soil Carbon and Nitrogen Dynamics、Genetic and phenotypic traits in livestock

Background/Objective Silage characteristics of grass materials directly affect their silage qualities. To expand the source of silage raw materials and develop mixed silages underlined by exploring the positive interactions between forage grasses and legumes, three gramineous grasses, Napier grass ( Pennisetum purpureum ), king grass ( Pennisetum sinese ), and forage maize ( Zea mays ) were separately mixed ensiled with a combination of four forage legumes including Medicago sativa , Vicia villosa , Vicia sativa , and Trifolium repens . Methods The chemical composition and fermentation quality of the mixed silages were analyzed and compared with those of the sole silages of these three grasses, as well as the diversity of microbial communities, through the 16S/ITS full-length sequencing. Results The results showed that the inclusion of forage legumes could somewhat improve the fermentation quality, as indicated by significantly ( p < 0.05) higher crude protein and lactic acid contents while lower neutral detergent fiber, acid detergent fiber contents and pH values, compared with the sole silages. Among the three types of mixed silages, the mixed king grass had the highest dry matter and crude protein content as well as lowest neutral detergent fiber and acid detergent fiber content. Meanwhile, the bacterial and fungal communities in the mixed silages were influenced by increased the relative abundance of lactic acid bacteria, which inhibited the proliferation of undesi...