Comparative Proteomic Analysis of <i>Campylobacter jejuni</i> Cultured at 37ºC and 42ºC

作者：Mao-Jun Zhang, Di Xiao, Fei-Li Zhao, Yixin Gu, Fanliang Meng, Lihua He, Guang-Yuan Ma, Jian-Zhong Zhang · 发表于：Japanese Journal of Infectious Diseases · 年份：2009 · DOI：10.7883/yoken.jjid.2009.356 · 被引用次数：29 · 研究领域：Salmonella and Campylobacter epidemiology、Aquaculture disease management and microbiota、Bacteriophages and microbial interactions

Campylobacter jejuni has the potential to thrive at 37ºC (e.g., in the human intestinal tract) and 42ºC (e.g., in the poultry intestinal tract). We aimed to determine the protein expression profiles of C. jejuni cultured at 37ºC and 42ºC in vitro by two-dimensional electrophoresis (2DE). The differentially expressed spots/proteins between C. jejuni cultured at 37ºC and 42ºC were defined when their expression differed by twofold. The differently expressed spots detected from C. jejuni cultured both on agar and in broth at 37ºC and 42ºC were subjected to protein identification by MALDI TOF/TOF. Overall, 15 and 20 differentially expressed proteins were defined for C. jejuni cultured at the two temperatures on agar and in broth, respectively. All of the identified differentially expressed proteins could be clustered as proteins involving the metabolism, regulator system, periplasmic proteins and the major antigens of C. jejuni. In conclusion, there are subsets of proteins that are optimally expressed at 37ºC, which may contribute to the host adaptation and/or the pathogenicity in the human intestinal tract.