Isolation, identification, and pathogenicity analysis of newly emerging gosling astrovirus in South China

作者：Jingyu Xu, Liguo Gao, Puduo Zhu, Sheng Chen, Zixian Chen, Zhuanqiang Yan, Wencheng Lin, Lijuan Yin, Muhammad Tariq Javed, Zhaoxin Tang, Feng Chen · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1112245 · 被引用次数：20 · 研究领域：Viral gastroenteritis research and epidemiology、Animal Virus Infections Studies、Viral Infections and Immunology Research

Goose astroviruses (GoAstV) cause fatal gout and decrease product performance in the waterfowl industry across the world. Since no effective vaccines are available, studies on the epidemiology of the virus are necessary for vaccine development. In this study, we collected 94 gout samples from goose farms in the Guangdong Province of South China. Among them, 87 samples (92.6%) tested positive for GoAstV, out of which five GoAstV strains were isolated after four generations of blind transmission through healthy 13-day-old goose embryos. The whole genome of the isolates was sequenced and further analyzed by comparing the sequences with published sequences from China and other parts of the world. The results of the alignment analysis showed that nucleotide sequence similarities among the five GoAstV isolates were around 97.4-98.8%, 98.6-100%, 98.1-99.8%, and 96.7-100% for the whole genome, ORF1a, ORF1b, and ORF2, respectively. These results showed that the GoAstV isolates were highly similar to each other, although they were prevalent in five different regions of the Guangdong Province. The results of the phylogenetic analysis showed that the whole genome, along with the ORF1a, ORF1b, and ORF2 genes of the isolates, were clustered on a single branch, along with the recently published GoAstV-2, and were very distinct from the DNA sequences of the GoAstV-1 virus. In this study, we also reproduced the clinical symptoms of natural infection using the GoAstV-GD2101 isolates, confirmin...