The effect of growth hormone on the metabolome of follicular fluid in patients with diminished ovarian reserve

作者：Fan He, Fang Wang, Yang Yang, Yuan Zhi, Cheng‐Guang Sun, Heng Zou, Huijia Chen, Hongliang Yi, Shan Hu Gao, Shen Zhang, Lina Hu, Ting­-Li Han · 发表于：Reproductive Biology and Endocrinology · 年份：2023 · DOI：10.1186/s12958-023-01073-x · 被引用次数：17 · 研究领域：Ovarian function and disorders、Reproductive Biology and Fertility、Endometriosis Research and Treatment

BACKGROUND: Increasing evidence supports that the co-treatment with growth hormone (GH) enhances ovarian response and oocyte quality during controlled ovarian stimulation (COS) in patients with diminished ovarian reserve (DOR). The composition of follicular fluid (FF) plays an essential role in oocyte development and mirrors the communication occurring between the oocyte and follicular microenvironment. However, the effect of GH on the FF metabolome remains unclear. METHODS: This prospective observational study recruited DOR patients undergoing in vitro fertilization (IVF) cycles with minimal stimulation protocol for COS. Each patient receiving GH co-treatment was matched to a patient without GH co-treatment by propensity score matching. The FF was collected after isolating oocytes and assayed by gas chromatograph-mass spectrometry (GC-MS) metabolomics. The Pearson correlation was performed to evaluate the relationship between the number of oocytes retrieved and the levels of differential metabolites. The KEGG database was used to map differential metabolites onto various metabolic pathways. RESULTS: One hundred thirty-four FF metabolites were identified by GC-MS metabolomics. Twenty-four metabolites, including glutathione, itaconic acid and S-adenosylmethionin (SAM) showed significant differences between the GH and control groups (p-value < 0.05 and q-value < 0.1). In addition, the number of oocytes retrieved was significantly higher in the GH group compared to the control g...