Systematic Enzymatic Synthesis of dTDP‐Activated Sugar Nucleotides

作者：Fangyu Wei, Rui Yuan, Wen Qin, Liuqing Wen · 发表于：Angewandte Chemie International Edition · 年份：2023 · DOI：10.1002/anie.202217894 · 被引用次数：11 · 研究领域：Carbohydrate Chemistry and Synthesis、Glycosylation and Glycoproteins Research、Microbial Metabolites in Food Biotechnology

Deoxythymidine diphosphate (dTDP)-activated sugar nucleotides are the most diverse sugar nucleotides in nature. They serve as the glycosylation donors of glycosyltransferases to produce various carbohydrate structures in living organisms. However, most of the dTDP-sugars are difficult to obtain due to synthetic difficulties. The limited availability of dTDP-sugars has hindered progress in investigating the biosynthesis of carbohydrates and exploring new glycosyltransferases in nature. In this study, based on the de novo and salvage biosynthetic pathways, a variety of dTDP-activated sugar nucleotides were successfully prepared in high yields and on a large scale from readily available starting materials. The produced sugar nucleotides could provide effective tools for fundamental research in glycoscience.