Transposable element and host silencing activity in gigantic genomes

作者：Jie Wang, Liang Yuan, Jiaxing Tang, Jiongyu Liu, Cheng Sun, Michael W. Itgen, Guiying Chen, Stanley K. Sessions, Guangpu Zhang, Rachel Lockridge Mueller · 发表于：Frontiers in Cell and Developmental Biology · 年份：2023 · DOI：10.3389/fcell.2023.1124374 · 被引用次数：14 · 研究领域：Chromosomal and Genetic Variations、Genomics and Phylogenetic Studies、CRISPR and Genetic Engineering

Transposable elements (TEs) and the silencing machinery of their hosts are engaged in a germline arms-race dynamic that shapes TE accumulation and, therefore, genome size. In animal species with extremely large genomes (>10 Gb), TE accumulation has been pushed to the extreme, prompting the question of whether TE silencing also deviates from typical conditions. To address this question, we characterize TE silencing via two pathways—the piRNA pathway and KRAB-ZFP transcriptional repression—in the male and female gonads of Ranodon sibiricus , a salamander species with a ∼21 Gb genome. We quantify 1) genomic TE diversity, 2) TE expression, and 3) small RNA expression and find a significant relationship between the expression of piRNAs and TEs they target for silencing in both ovaries and testes. We also quantified TE silencing pathway gene expression in R. sibiricus and 14 other vertebrates with genome sizes ranging from 1 to 130 Gb and find no association between pathway expression and genome size. Taken together, our results reveal that the gigantic R. sibiricus genome includes at least 19 putatively active TE superfamilies, all of which are targeted by the piRNA pathway in proportion to their expression levels, suggesting comprehensive piRNA-mediated silencing. Testes have higher TE expression than ovaries, suggesting that they may contribute more to the species’ high genomic TE load. We posit that apparently conflicting interpretations of TE silencing and genomic gigan...