High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq

作者：Yang Liu, Marcello DiStasio, Graham Su, Hiromitsu Asashima, Archibald Enninful, Xiaoyu Qin, Yanxiang Deng, Jungmin Nam, Fu Qiang Gao, Pino Bordignon, Marco Cassano, Mary M. Tomayko, Mina L. Xu, Stephanie Halene, Joseph E. Craft, David A. Hafler, Rong Liang Fan · 发表于：Nature Biotechnology · 年份：2023 · DOI：10.1038/s41587-023-01676-0 · 被引用次数：413 · 研究领域：Single-cell and spatial transcriptomics、Immunotherapy and Immune Responses、Immune cells in cancer

In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and demonstrated high-plex protein and whole transcriptome co-mapping. We profiled 189 proteins and whole transcriptome in multiple mouse tissue types with spatial CITE sequencing and then further applied the method to measure 273 proteins and transcriptome in human tissues, revealing spatially distinct germinal center reactions in tonsil and early immune activation in skin at the Coronavirus Disease 2019 mRNA vaccine injection site.