A novel mechanism of cannabidiol in suppressing ovarian cancer through LAIR ‐1 mediated mitochondrial dysfunction and apoptosis

作者：Li Ma, Huachang Zhang, Chuntong Liu, Mengke Liu, Fugen Shangguan, Yan Liu, Shude Yang, Hua Li, Jing An, Shuling Song, Qizhi Cao, Guiwu Qu · 发表于：Environmental Toxicology · 年份：2023 · DOI：10.1002/tox.23752 · 被引用次数：23 · 研究领域：Cannabis and Cannabinoid Research、Diet, Metabolism, and Disease、Pancreatic function and diabetes

Cannabidiol (CBD) is a nonpsychoactive cannabinoid compound. It has been shown that CBD can inhibit the proliferation of ovarian cancer cells, but the underlying specific mechanism is unclear. We previously presented the first evidence for the expression of leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1), a member of the immunosuppressive receptor family, in ovarian cancer cells. In the present study, we investigated the mechanism by which CBD inhibits the growth of SKOV3 and CAOV3 ovarian cancer cells, and we sought to understand the concurrent role of LAIR-1. In addition to inducing ovarian cancer cell cycle arrest and promoting cell apoptosis, CBD treatment significantly affected the expression of LAIR-1 and inhibited the PI3K/AKT/mTOR signaling axis and mitochondrial respiration in ovarian cancer cells. These changes were accompanied by an increase in ROS, loss of mitochondrial membrane potential, and suppression of mitochondrial respiration and aerobic glycolysis, thereby inducing abnormal or disturbed metabolism and reducing ATP production. A combined treatment with N-acetyl-l-cysteine and CBD indicated that a reduction in ROS production would restore PI3K/AKT/mTOR pathway signaling and ovarian cancer cell proliferation. We subsequently confirmed that the inhibitory effect of CBD on the PI3K/AKT/mTOR signal axis and mitochondrial bioenergy metabolism was attenuated by knockdown of LAIR-1. Our animal studies further support the in vivo anti-tumor activity of...