Two doses of Lactobacillus induced different microbiota profiles and serum immune indices in pigs

作者：Dongyan Zhang, Sixin Wang, Guixia Li, Meixia Chen, Hui Liu, Haifeng Ji · 发表于：Journal of Functional Foods · 年份：2023 · DOI：10.1016/j.jff.2023.105405 · 被引用次数：18 · 研究领域：Gut microbiota and health、Probiotics and Fermented Foods、Animal Nutrition and Physiology

We evaluated the changes in microbiota profiles and serum immune indices of growing pigs after dietary supplementation with two different doses of Lactobacillus reuteri ZLR003. Both Lactobacillus doses significantly affected the growth performance of pigs. Copy number/g feces showed that L. reuteri ZLR003 reached the gastrointestinal tract and accumulated, but the higher dose was excreted more. L. reuteri ZLR003 increased species richness and Bacteroidetes abundance. The abundances of Clostridium_sensu_stricto_1, Terrisporobacter, Blautia, Corynebacterium and Streptococcus were significantly lower, and those of Christensenellaceae_R-7_group, Rikenellaceae_RC9_gut_group, Bifidobacteriaceaeg_Bifidobacterium and Lactobacillus were significantly higher in both Lactobacillus-treated groups than in controls. Abundances of Clostridium_sensu_stricto_1, Terrisporobacter and Lactobacillus differed significantly between the high- and low-dose Lactobacillus-treated groups. The acetic, butyric, propionic, valeric and isovaleric acid concentrations were higher in both Lactobacillus-treated groups than in controls, and acetic and butyric acid concentrations were higher in the low-dose group than in the high-dose group. The Lactobacillus-treated pigs had increased serum immunoglobin G, immunoglobin M, interferon-γ, interleukin (IL)-2, IL-4 and IL-10 levels and a decreased tumor necrosis factor-α concentration compared with the control group. Serum levels of interferon-γ were increased, and s...