Can biomarkers identified from the uterine fluid transcriptome be used to establish a noninvasive endometrial receptivity prediction tool? A proof-of-concept study

作者：Aihua He, Hong Wu, Yangyun Zou, Cheng Wan, Jing Zhao, Qiong Zhang, Nenghui Liu, Donge Liu, Yumei Li, Yumei Li, Jing Fu, Hui Li, Xi Huang, Tianli Yang, Chunxu Hu, Zhaojuan Hou, Yue Sun, Xin Dong, Sijia Lü, Sijia Lü, Yanping Li, Yanping Li · 发表于：Reproductive Biology and Endocrinology · 年份：2023 · DOI：10.1186/s12958-023-01070-0 · 被引用次数：16 · 研究领域：Reproductive System and Pregnancy、Ovarian function and disorders、Gynecological conditions and treatments

BACKGROUND: Embryo implantation in a receptive endometrium is crucial for successful pregnancy. Endometrial receptivity (ER) prediction tools based on endometrial transcriptome biomarkers by endometrial biopsy have been used to guide successful embryo implantation in in vitro fertilization (IVF) patients. However, no reliable noninvasive ER prediction method has been established, and one is greatly needed. We aimed to identify biomarkers from uterine fluid transcriptomic sequencing data for establishing noninvasive ER prediction tool and to evaluate its clinical application potential in patients undergoing IVF. METHODS: The non-invasive RNA-seq based endometrial receptivity test (nirsERT) was established by analyzing transcriptomic profile of 144 uterine fluid specimens (LH + 5, LH + 7, and LH + 9) at three different receptive status from 48 IVF patients with normal ER in combination with random forest algorithm. Subsequently, 22 IVF patients who underwent frozen-thaw blastocyst transfer were recruited and analyzed the correlation between the predicted results of nirsERT and pregnancy outcomes. RESULTS: A total of 864 ER-associated differentially expressed genes (DEGs) involved in biological processes associated with endometrium-embryo crosstalk, including protein binding, signal reception and transduction, biomacromolecule transport and cell-cell adherens junctions, were selected. Subsequently, a nirsERT model consisting of 87 markers and 3 hub genes was established using a ...