Multi-omic approach associates blood methylome with bronchodilator drug response in pediatric asthma

作者：Javier Pérez-García, Esther Herrera‐Luis, Annie Li, Angel C. Y. Mak, Scott Huntsman, Sam S. Oh, Jennifer R. Elhawary, Celeste S. Eng, Kenneth B. Beckman, Donglei Hu, Fabián Lorenzo-Díaz, Michael A. LeNoir, José Rodríguez‐Santana, Noah Zaitlen, Jesús Villar, Luisa N. Borrell, Esteban G. Burchard, Maria del Mar Pino-Yanes · 发表于：Journal of Allergy and Clinical Immunology · 年份：2023 · DOI：10.1016/j.jaci.2023.01.026 · 被引用次数：19 · 研究领域：Asthma and respiratory diseases、Epigenetics and DNA Methylation、Delphi Technique in Research

BACKGROUND: Albuterol is the drug most widely used as asthma treatment among African Americans despite having a lower bronchodilator drug response (BDR) than other populations. Although BDR is affected by gene and environmental factors, the influence of DNA methylation is unknown. OBJECTIVE: This study aimed to identify epigenetic markers in whole blood associated with BDR, study their functional consequences by multi-omic integration, and assess their clinical applicability in admixed populations with a high asthma burden. METHODS: We studied 414 children and young adults (8-21 years old) with asthma in a discovery and replication design. We performed an epigenome-wide association study on 221 African Americans and replicated the results on 193 Latinos. Functional consequences were assessed by integrating epigenomics with genomics, transcriptomics, and environmental exposure data. Machine learning was used to develop a panel of epigenetic markers to classify treatment response. RESULTS: ). Moreover, a panel of 70 CpGs showed good classification for those with response and nonresponse to albuterol therapy in African American and Latino children (area under the receiver operating characteristic curve for training, 0.99; for validation, 0.70-0.71). The DNA methylation model showed similar discrimination as clinical predictors (P > .05). CONCLUSIONS: We report novel associations of epigenetic markers with BDR in pediatric asthma and demonstrate for the first time the applicabili...